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KMID : 0364820230590020069
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Korean Journal of Microbiology
2023 Volume.59 No. 2 p.69 ~ p.76
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Phreatobacter aquaticus sp. nov., isolated from freshwater
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Choi Ah-Young
Chung Eu-Jin
Ryu Shi-Hyun
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Abstract
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A novel bacterial strain, designated NMCR1094T, was isolated from the freshwater of the Oship stream, Republic of Korea.
Cells of the strain were Gram-stain-negative, aerobic, rod-shaped and motile by means of a polar flagellum. Growth was observed at 10?40°C (optimum, 25°C), pH 6.5?10.0 (optimum 7.0) and 0?0.5 % (w/v; optimum at 0% NaCl) on R2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain NMCR1094T belonged to the genus Phreatobacter and shared 98.4?98.6% sequence similarities with Phreatobacter species. The complete genome size of the strain NMCR1094T is 4,975,052 bp, with G + C content of 67.6 mol%. Average nucleotide identity and digital DNA-DNA hybridisation values were 78.3?78.5% and 20.1?20.5%, respectively between strain NMCR1094T and the Phreatobacter species. The major respiratory quinone found in strain NMCR1094T was ubiquinone-10. The major fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C18:1 ω7c 11-methyl. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, six unidentified glycolipids, three unidentified phospholipids and an unidentified aminolipid. Phenotypic, phylogenetic, genomic and chemotaxonomic characteristics showed that strain NMCR1094T represents a novel species of the genus Phreatobacter, for which the name Phreatobacter aquaticus sp. nov. is proposed. The type strain is NMCR1094T (= KACC 19706T = NBRC 113394T = FBCCB10017).
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KEYWORD
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Phreatobacter aquaticus, novel species, polyphasic taxonomy
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